Next-Gen Intelligent TechBio

We enable patient-derived epithelial organoids for high-throughput, multi-modal genetics-proteomics-histology phenotypic profiling, revolutionizing biopharma and medicare with unleased AI.

Alvus Partnered with
NASA+MGH+SpaceTango
‍ to send PDO to SPACE
In March 2024

NASA sent our organoids to space

Next-Gen Intelligent TechBio

We enable patient-derived epithelial organoids for high-throughput, multi-modal phenotypic profiling, revolutionizing biopharmaceutical discovery with unleased AI.

Alvus+ NASA+MGH
‍ sent PDO to space

Alvus Partnered with NASA+MGH+SpaceTango
to send PDO to SPACE in March 2024

Alvus is trusted and working with incredible teams:

A Surrogate Human Epithelial Organ System

Capitalizing on the breakthrough with GI epithelial organoids, Alvus is diversifying its surrogate organ biobank, preserving the molecular, structural, and functional characteristics of their origins. We leverage our insights into biophysical properties and disease mechanisms across various human epithelial tissues.

Intestinal

Lung

Stomach

Cerebral

Urinary

Ovarian

Enables new era of rapid, low-cost high throughput screening and identification

Alvus's organoids merge the benefits of cell lines and 3D organoids.

Cell Line

3D Organoid

Alvus Health

Physiology

Low

High

High

Throughput

High

Low

High

Cost efficiency

High

Low

High

Standarization

High

Low

High

We can collect various human organoids

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Intestinal

Lung

Stomach

Cerebral

Urinary

Ovarian

Enables new era of rapid, low-cost high throughput screening

Our organoid culturingtakes 1 week and 20% cost. Our model combines the advantages of both cell line and 3D organoids.

Physiological relevance

Cell line

2D

Low

Disadvantages:

No any info in organ level

Organoinds

3D

Medium

Disadvantages:

Loss of open-lumen anatomy
Artifical pressure
Short term culture
Dead cell accumulation

Alvus Health

2.5D

High

Throughput capacity

Cost efficiency

High

High

Low

Disadvantages:

Long time commitment
Custom-made media
High cost of Matrigel

Low

Disadvantages:

Heterogeneity
Tedious protocol
Difficult imaging process
Variation in Matrigel

High

Medium

Cell line

2D

Organoinds

3D

Alvus Health

2.5D

Physiological relevance

Low

Disadvantages:

No any info in organ level

Medium

Disadvantages:

Loss of open-lumen anatomy
Artifical pressure
Short term culture
Dead cell accumulation

High

Throughput capacity

High

Low

Disadvantages:

Long time commitment
Custom-made media
High cost of Matrigel

High

Cost efficiency

High

Low

Disadvantages:

Heterogeneity
Tedious protocol
Difficult imaging process
Variation in Matrigel

Medium

Automation and Screening

Alvus's organoids easily integrate with high-throughput automation, facilitate cell transfection for gene editing, and streamline imaging data collection.

01

02

03

04

Organoids culture

Organoids culture

Organoids culture

Organoids culture

01

02

03

04

Organoids culture

Organoids culture

Organoids culture

Organoids culture

Automation and Screening

Alvus's organoids easily integrate with high-throughput automation, facilitate cell transfection for gene editing, and streamline imaging data collection.

01

02

03

04

Organoids culture

Organoids culture

Organoids culture

Organoids culture

01

02

03

04

Organoids culture

Organoids culture

Organoids culture

Organoids culture

Automation and Screening

Alvus's organoids easily integrate with high-throughput automation, facilitate cell transfection for gene editing, and streamline imaging data collection.

01

02

03

04

Organoids culture

Organoids culture

Organoids culture

Organoids culture

01

02

03

04

Organoids culture

Organoids culture

Organoids culture

Organoids culture

High-throughput automated organoid culturing and AI-enabled phenotypic analysis optimizes and amplifies intricate biological research

Collaborating with pioneering centers advancing lab automation, such as the Harvard ICCB-Longwood Screening Facility, Alvus harnesses high-throughput organoid screening to explore small molecules and functional genomics.

Healthy

Crypt-villus morphology via nuclear staining

CRC

Crypt-villus morphology via nuclear staining

Healthy

Permeability via ZO-1

Healthy

Stem cells via LGR5

Scalable Data:
All-round Phenotypic Profiling via Multiplex Staining and Sequencing

Phenotypic assays monitor the establishment of cell morphology and phenotype of interest in a target-agnostic manner, enabling the exploration of vast biological space. By leveraging advanced multiplex imaging techniques, we can profile the molecular characteristics of organoids at the single-cell level.

CRC

Permeability via ZO-1

Healthy

Goblet cells  via MUC-2

CRC

Enterocytes via ALPI

Healthy

Enterocytes via ALPI

CRC

Goblet cells  via MUC-2

CRC

Stem cells via LGR5

Healthy

Crypt-villus morphology via nuclear staining

CRC

Crypt-villus morphology via nuclear staining

Healthy

Permeability via ZO-1 staining

CRC

Permeability via ZO-1 staining

Healthy

Stem cells via LGR5 staining

CRC

Crypt-villus morphology via nuclear staining

Healthy

Crypt-villus morphology via nuclear staining

Scalable Data:
All-round Phenotypic Profiling via Multiplex Staining and Sequencing

Phenotypic assays monitor the establishment of cell morphology and phenotype of interest in a target-agnostic manner, enabling the exploration of vast biological space. By leveraging advanced multiplex imaging techniques, we can profile the molecular characteristics of organoids at the single-cell level.

Healthy

Stem cells via LGR5 staining

CRC

Permeability via ZO-1 staining

Healthy

Crypt-villus morphology via nuclear staining

CRC

Crypt-villus morphology via nuclear staining

Healthy

Permeability via ZO-1 staining

CRC

Permeability via ZO-1 staining

Healthy

Stem cells via LGR5 staining

Contact

info@alvushealth.com

Pagliuca Harvard Life Lab
127 Western Avenue, Allston, MA, 02134

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